HomeHuman and murine platelets from whole blood can be immunophenotyped by the labelling of GpIb and GpIIb/IIIa to determine the presence of the respective clinical syndromes Glanzmann's Thrombasthenia and Bernard-Soulier syndrome and also the counting of platelet numbers.
The homozygote double recessive and heterozygote conditions of Glanzmann's Thrombasthenia has a >90% and approximately 50% reduced expression of GpIIb/IIIa respectively. Bernard-Soulier's syndrome showing a marked reduction in GpIb expression that can be readily shown by flow cytometric analysis after immunophenotyping of GpIb.
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